Journal articles: 'Zong jiao' – Grafiati (2024)

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Educational exchange between Malaysia and China has an important interaction with the Chinese education in Malaysia and the political relations between Malaysia and China. Chinese education in Malaysia is twisted with Malaysian ethnic politics. Malaysian Chinese students were lack of opportunities at the public institutions of higher learning due to the preferential and affirmative action policies implemented by Malaysian government under the egis of New Economic Policy (NEP).Thus, Malaysia NGOs (Non-Governmental Organizations) carried out educational exchange with China to open up a more spacious study channels for students. The objective of this article is to investigate the role of Malaysian NGOs towards educational exchange between Malaysia and China under Malaysian ethnic politics. Primary data were collected through interviews and the informants were selected based on purposive sampling and snowball sampling. Totally, fourteen informants were selected and interviewed based on evidence of data saturation. Secondary data were collected from journal articles, newspapers, website pages and online resources. Meanwhile, structuration theory was used to guide the researcher to get the suitable answer. Findings of this study indicate that, there are three main important NGOs that play a significant role in promoting educational exchange between Malaysia and China, which are Dong Jiao Zong (the collective name of Dong Zong and Jiao Zong), the Association of Graduates from Universities and Colleges in China, Malaysia (AGUCCM) and Malaysia-China Friendship Association (MCFA). As the purposive actors, they conduct their actions based on different levels, which are to develop Chinese education in Malaysia, to continue and inherit Chinese culture. Their unconsciousness reason is based on Malaysian Chinese’s deep feeling to China. Moreover, the educational exchange in the aspect of Chinese education is conducted under the structure of Malaysian ethnic politics, which led to a dichotomy. Nowadays, with the rise of China and the promotion of “the Belt and Road”, educational exchange between Malaysia and China will become better and better.

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Background: To explore the prescription rules of famous ancient physicians in the treatment of threatened miscarriage. Methods: Traditional Chinese Medicine (TCM) prescriptions for threatened miscarriage were screened out of Fu Ren Da Quan Liang Fang by Ziming Chen, Yi Zong Jin Jian by Qian Wu, and Fu Qing Zhu Nv Ke by Qingzhu Fu. Data were standardized and analyzed through the TCM inheritance auxiliary platform. Results: A total of 29 prescriptions for threatened miscarriage were screened. Dang Gui, E Jiao, Gan Cao, Chuan Xiong, Bai Shao were the top five frequently prescribed Chinese herbs. The common herb–herb combinations used by Ziming Chen contained E Jiao, Dang Gui, Chuan Xiong, Ai Ye, Cong Bai, and Sang Ji Sheng. Ren Shen, Gan Cao, and Bai Zhu were the common herbal groups used by Qingzhu Fu. Huang Qi, Shu Di Huang, Bai Shao, Dang Gui, and Gan Cao were one of Qian Wu’s core prescriptions, with Dang Gui and Chuan Xiong being the others. According to the analysis of four Qi, five flavors, and meridian tropism of the prescriptions, herbs with the warm nature, or with the sweet, pungent, bitter flavors topped the list of application. The top six meridian tropisms of high-frequency herbs were: liver, spleen, lung, kidney, heart, and stomach meridian. Conclusion: Based on the principle of restoring the balance within the organs and enriching Qi and blood, clinical treatment of threatened miscarriage involves invigorating the Chong and Ren channels, nourishing Yin, dispelling cold and wind, generating and activating blood, regulating and harmonizing Qi.

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Bayat adalah salah satu daerah di Pulau Jawa yang memiliki singkapan batuan pra-Tersier. Batuan terkekarkan karena aktivitas tektonik. Hal ini membutuhkan penjelasan dan analisis terperinci untuk memahami zona sesar dan mekanismenya. Penelitian dilakukan untuk mengetahui zona sesar dan karakteristiknya di Perbukitan Jiwo Barat, Kecamatan Bayat, Kabupaten Klaten, Jawa Tengah, dan juga mekanisme pembentukan zona sesar untuk mengetahui kerangka tektoniknya. Metode penelitian ini meliputi analisis topografi, analisis petrografi, proyeksi stereografi, dan penentuan kronostratigrafi. Proyeksi stereografi memanfaatkan data hasil pengukurn struktur lapangan dan dijalankan pada perangkat lunak Dips. Hasil analisis dipakai untuk mendukung interpretasi tentang mekanisme pembentukan zona sesar di daerah penelitian. Stratigrafi di lokasi penelitian, dari urutan tua ke muda, terdiri dari Batuan Metamorf, Batupasir, Batugamping Eosen, Intrusi Batuan Beku, Batugamping Miosen, dan Aluvial. Zona sesar yang ditemukan di daerah penelitian adalah sesar mendatar menganan yang terdiri dari Sesar Mendatar Tugu, Sesar Mendatar Sari dan Sesar Mendatar Kebo, dan detachment fault yang terdiri dari Jowo Detachment Fault. Zona sesar memiliki dua orientasi utama, barat daya-timurlaut (N 217o E) dan barat laut-tenggara (N 330o E, N 122o E, dan N 287o E). Mekanisme pembentukan zona sesar di lokasi penelitian terjadi dalam dua periode tektonik, pada Kapur Akhir karena subduksi baratdaya - baratlaut yang membentuk Sesar Mendatar Tugu dan Sesar Mendatar Kebo, dan pada Akhir Eosen-Oligosen yang disebabkan oleh subduksi utara-selatan yang membentuk Sesar Mendatar Sari dan Jowo Detachment Fault.

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AbstractThe Benxi area in Liaoning Province is one of the most important iron-ore districts in China. This study uses nonseismic geophysical data (in the form of gravity–magnetic–magnetotelluric data) and based on the section modeling method to model the deep, three-dimensional geological structure of the Benxi area. Based on the modeling results and deep geological structure characteristics, the Benxi area can be divided into three first-order deep geological tectonic units. A close relationship is between tectonic unit and iron-ore concentrations. First, high-quality iron-ore deposits occur within the tectonic boundary and sedimentary boundary zone of the Jiao–Liao–Ji Belt, reflects the protective effect of sedimentary cover on the iron-bearing formation. Second, enriched iron-ore deposits are mainly developed in Mesozoic granitic intrusion zone, reflects the hydrothermal leaching of silicon in host iron-bearing formations during magma intrusion. Thus, the findings of this study have important implications for future prospecting in the Benxi iron-ore district.

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Background:Juvenile idiopathicarthritis (JIA) comprises a group of inflammatory diseases that frequently requires systemic treatments. There are some studies that evaluate the systemic drug survival in adults with JIA; but there is scarce data about the drug survival in paediatric population.Objectives:Our main objective was to study the drug survival of biologic therapies and synthetic DMARD in a monocentric cohort and the related factors influencing on it.Methods:Patients with JIA visited in the last 12 years were included. We carried out a retrospective, longitudinal study and collected data on treatment (start date, tapering and stop the treatment date; causes of finish and combined treatment or not). We also collected demographic data with date of birth, sex, symptoms onset data and JIA subcategory. We studied time to relapse since the drug suspension. The drug survival for each kind of treatment was analyzed with Kapplan-Meier curves.Results:We included 158 patients with JIA. Demographic data are shown in table 1. One hundred and thirty (82.3%) patients started methotrexate (MTX) with a half-life of 34.8 months; 79 (51.5%) patients started biologic therapy with half-life of 29 months and 14 (17.7%) patients started a second biologic with a half-life of 5 months. Time to first tapering of MTX was 12 months, for the first biologic was 10.5 months and for the second biologic was of 15 months. The main cause of suspension was remission for each group. Treatment according to different JIA subcategories is shown in table 2. In 45 patients (28.5%) systemic treatment was stopped and 11 (24.4%) had a disease flare in a mean time of 36 months. Taking into account only patients who flared, the mean time was 15.6 months.Table 1.Overview of patients with BSLE. BMZ: basal membrane zone MTP: methylprednisolone DDS: dapsoneTable 1.Demographic features of the patients.Total (n:158)Sex (female)96 (60,76%)Starting age (median y RI)4,9 (2,6-9,8)AAN positives91 (57,6%)JIA oligo persistent66 (41,77%)JIA oligo extended16 (10,13%)JIA enthesitis14 (8,86%)JIA psoriasic9 (5,7%)JIA systemic18 (11,39%)JIA poli FR-29 (18,35%)JIA poli FR+4 (2,53%)JIA undifferentiated2 (1,27%)Table 2.All diseases that are diagnosed in patients during the follow-up period.Group 1 (Systemic JIA n=18 (%)Group 2 (Oligoarticular JIA n=82 (%)Group 3 (Poliarticular JIA n=33 (%)Group 4 (Juvenil SpA n=23 (%)Synthetic DMARD(n:130)9 (50%)71 (86,6%)33 (100%)17 (74%)Biologic Therapy(n:79)6 (33,3%)34 (41,46%)22 (75%)17 (74%)No systemic treatment(n:21)7 (38,9%)10 (12,2%)04 (17.4%)Fig 1.Histology and DIF of BSLE H-Eaandb: subepidermal blisterscandd: abundant neutrophils are observede: DIF full house linear patternFig 2.skin lesions of BSLEa: tense serous blisters, scars and erosionsb: purpuric macules in palmsc: blisters in groind-g: blisters and hypopigmented scarsConclusion:The drug survival for systemic therapies in children with JIA is more than 2 years, without significant differences between synthetic DMARDs and biologics. Remission is the main cause for ending treatment. Biologic drug survival was significantly shorter between systemic JIA and the other subcategories. Only one fourth of patients had a flared after stopping the systemic treatment.Disclosure of Interests:Laura Trives Folguera Speakers bureau: ROCHE, Indalecio Monteagudo: None declared, Belén Serrano Benavente: None declared, Liz R. Caballero Motta: None declared, Ana Melissa Anzola: None declared, Katerine López Gloria: None declared, Juan Carlos Nieto Speakers bureau: Pfizer, Abbvie, MSD, Novartis, Janssen, Lilly, Nordic Pharma, BMS, Gebro, FAES Farma, Roche, Sanofi

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The wooden shan-jia structure (The braced arch structure), the main load-bearing structure in the traditional tile board room of Yi minority, is one of the residential elements which are most distinctive and most valuable to be protected. As the traditional architectural culture of the Yi, the structure is still being widely used in local residence, but the Liangshan Yi Minority is located in high intensity earthquake zone, which cause large security risks on the wooden frame system. In this paper, three kinds of common and typical layouts of braced arch structure are collected after investigating of the wooden shan-jia structure. The structural weaknesses are found through the simulation of ANSYS. Finally, propose some measures to optimize the structural design, in order to enhance the seismic optimization and improve the construction technology of tile board room.

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Fault fracture zone with rich water has some bad geological conditions such as low strength, deformation, strong water permeability, poor water resistance. This paper is based on Yan-jia Tunnel in HuBei-Guzhu Highway. WSS is taken as the advanced support method and has improved the construction condition. the construction method of meshshotcreting firstly is adopted. The result of numerical simulation with ABAQUS is basically consistent with the field measurements, which has significance on the subsequent design and construction of underground projects with similar geological conditions.

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Fault fracture zone with rich water has some bad geological conditions such as low strength, deformation, strong water permeability, poor water resistance. Landslide has been happened because the water permeability. This paper is based on Yan-jia Tunnel in HuBei-Guzhu Highway. For the bad geological conditions, WSS is taken as the advanced support method to reinforce soil and stop watering. The method has improved the construction condition. To reduce the excavation area in one time and the disturbance to surrounding soil, the construction method of meshshotcreting firstly is adopted. The paper also do the numerical simulation with the numerical calculation tool of the software ABAQUS. The result is basically consistent with the field measurements, which has significance on the subsequent design and construction of underground projects with similar geological conditions

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The New Chinese Cinemas were unprecedented in critiquing official narratives of progress through dramatic location-shot images of rural Taiwan and China. Much more than standing in as a picturesque backdrop, the rural was a site of complex ideological contestations. Yet, existing scholarship overlooks the richness of rural representations, reductively interpreting rural films as works of nostalgia and cultural salvage. Through a comparative analysis of representations of landscape, travel and visual perception in Hou Hsiao-Hsien’s Dust in the Wind (1986) and Jia Zhangke’s Platform (2000), this article brings into focus the important but largely ignored roles that Hou and Jia have played in envisioning new frameworks for thinking about rural geographies. Drawing from Doreen Massey’s notion of the ‘progressive place’, I investigate how Jiufen and Fenyang – the films’ shooting locations – are stages upon which the directors experimented with imaging and imagining communities. Jiufen is represented in Dust as a porous interface between the urban and rural, a metonym for the film’s representation of Taiwan as a contact zone with China. Platform, by contrast, fashions an image of Fenyang as a non-place, a microcosm of China as it undergoes unchecked neo-liberal development. Significantly, these films went beyond revising rural imaginaries on-screen, to making a material impact on Jiufen and Fenyang by transforming them into landmarks of global film tourism. This work demonstrates how Hou and Jia responded to disorienting social changes not by resisting, but by tactically embracing the blurring of divides between the urban and rural, and local and global.

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The Nanpu 5th construct is in the western part of Huanghua Depression Nanpu Sag of Bohai Bay Basin, was a complicated anticline belt that develops between Jian Dong fault and the downthrown side of the southwestern Zhuang fault and the favorable exploration area is 120km2. On the basis of the region's large number of multi-channel seismic data analysis and interpretation, the trap types, structural characteristics and distribution of local structures between the layers of the region are researched. Interlayer local structures in the area are mainly divided into nose structure and small anticline. The fault zone is a structural high in the region, to promote oil and gas to migrate and accumulate to the low-potential zones that become favorable zones for hydrocarbon accumulation, but the real decisive construct parts of the hydrocarbon accumulation is positive local structure in favorable zones which point out the region for hydrocarbon accumulation.

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Bayat, Klaten, Central Java, is one of three locations in Java with complete types of rocks exposed at the earth’s surface. All those rocks are scattered over a short distance in Bayat, revealing past processes of rock deformation (folding, fracturing, and faulting) and present-day processes of rock weathering and erosion. In this study, we present how clastic carbonate rock of the Oyo Formation at northern Jiwo Hills could be separated about ±15 km northern from its platforms as an indication of thrust fault growth. This study uses aerial photography for photogrammetry (drones) combined with structural geology and microfossil analyses (to know the exact formation) from the outcrop observation. Recent studies have certified that drones are one reliable observation tool in various aspects with better resolution, especially in structural geology studies. Aerial photogrammetry is very well done to see the exact condition of a wide area combined with high resolution on an outcrop scale. The result shows that the carbonate rocks are from Oyo Formation (N9–N11) with the Middle Neritic bathymetric zone. The structural geology phenomenon kinematically indicates the impact of the transpressional movement called flower structure. Based on subsurface interpretation, the authors hypothesize this area was the product of an imbrication thrust stack uplifted basem*nt as the result of the thrust fault rather than horst or paleo-basem*nt high.

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Elucidating brine origin and evolution is a fundamental but not easy task especially for coastal geothermal systems with possible marine constituents and multistage evolution, as subsequently physical, chemical and biological alteration processes may mask the original and early-stage signatures. Here chemical and isotopic characteristics of water (D and 18O) and dissolved constituents (13C, 14C, 11B and 87Sr/86Sr) have been utilized to investigate the source and multistage evolution of the Jimo coastal geothermal system in eastern China, with dramatic differences of geochemical characteristics observed within a 0.2 km2 area. Results show that geothermal water is derived from paleo-meteoric water and has undergone a 3-stages evolution that involves: (1) Dissolution of marine halite and potash salts in the deep reservoir; (2) Water-rock reactions especially cation exchange produces a Cl-Na-Ca type water as deep geothermal water upwells along the fault zone; (3) A minor (<0.3%) addition of fossil seawater to the shallow aquifer that produces Cl-Na type waters in the west, whereas sulfide oxidation and dissolution of aluminosilicate and carbonates in the east produces Cl-Na-Ca type waters. The methodology utilized in this study offers a means of examining other similar complex geochemical systems having a multistage evolution.

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Magnetic sensors are widely used in areas such as high-density magnetic recording, navigation, military and security, target detection and tracking, anti-theft systems, non-destructive testing, magnetic marking and labeling, geomagnetic measurements, space research, measurements of magnetic fields onboard spacecraft and biomagnetic measurements in the body1. For magnetic sensors used to detect weak magnetic fields, a highly sensitive sensing element, with extremely high permeability, is needed. Permalloy is useful due to its initial high permeability and near-zero magnetostriction. Current fabrication methods used to produce these sensing elements include sputtering, electroplating, cold drawing and wet etching. Laser ablation, however, has always not been seriously considered due to domain pinning effects induced by nanosecond lasers2. Femtosecond laser machining, on the other hand, has shown great potential in processing such soft magnetic materials without affecting its permeability. In fact, Jia et al. had observed that when femtosecond laser micromachining was carried out on FINEMET, the amorphous phase was observed to remain in the damaged zone and few crystallization was found in the ablation zone3.

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Objective: to identify the features of modeling and give a morphofunctional assessment of the course of experimental myocardial infarction (EMI) in rats within the first 48 hours after ligation of the left coronary artery (LCA). Material and methods. EMI was reproduced according to the method of H. Selye [1] in Jian Ye`s modification [2] by means of LCA ligation in 24 Wistar rats. Electrocardiographical, histological, biochemical studies were performed. The control group consisted of 7 falsely operated animals in which the chest had been opened without manipulation on the LCA. Results. 5-15 minutes after LCA ligation, an ischemic change in the (Q)RST complex was observed as a monophasic curve. The Q wave appeared on the EGG after 1 hour of EMI in 20% cases, after 7 hours in 88% cases, in 100% cases after 27 hours and remained by 48 hours of EMI. Histologically, within the first 7 hours there was acute alterative myocardial damage followed by a quantitative increase in diffusely located necrotic cardiomyocytes (CMC) by 27 hours and the appearance of a clear zone of coagulation necrosis by 48 hours; which was accompanied by corresponding changes in the level of troponin I. Conclusion. The course of EIM after LCA ligation is characterized by the staging of myocardial changes similar to that in humans, which provides a prerequisite for possible extrapolation of the results of the studies to humans.

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The article deals with the central concept of Prajnaparamite philosophy – the concept of emptiness. The analysis of the understanding of this concept in the scientific literature is given, the difficulties of understanding it are indicated. Nagarjuna's explanations of the doctrine of emptiness are considered in the context of the Prajnaparamite theory of the middle. Much attention is paid to the difficulties of understanding emptiness in China during the formation of Buddhism in this country. The variants of understanding the concept of emptiness presented in the teachings of the "six schools and seven directions" (Liu jia qi zong) are considered. The interpretation of the understanding of emptiness in the writings of Seng Zhao and Kumarajiva is shown. The translation of the text of the Hridaya Sutra is presented, in which the definition of the concept of emptiness is given in a concise form. The object of the study is the philosophy of Prajnaparamita. The subject of the study is the interpretation of the concept of emptiness in the Prajnaparamite tradition. The scientific novelty of the article lies in the fact that it presents different points of view on the understanding of the concept of emptiness, both in the scientific literature and in the Buddhist tradition of India, Tibet and China. The main conclusion of the article is that the reason for the different interpretation of emptiness lies in the semantic features of the term "emptiness" which implies absolute negation. Therefore, there was a need to justify the fact that the negation is followed by a statement, the emptiness hides the true being. The next difficulty was the question of what truly exists. The search for the true essence of this being is especially clearly traced to the Chinese tradition.

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This paper describes the experimental parameters involved in the femtosecond laser micromachining of biodegradable poly(L-lactide) which is frequently used in biomedical applications such as vascular stents or scaffolds. We investigated the influence of laser pulse energy, scanning strategy and number of overscans on the laser cutting throughput. The process parameters that enable reducing of a heat affected zone were determined. As a result, the optimal scanning strategy was determined in order to obtain high aspect ratio trenches in 380 ?m thick biodegradable polymer sheet. Full Text: PDF ReferencesW. Jia et al. "Effects of high-repetition-rate femtosecond laser micromachining on the physical and chemical properties of polylactide (PLA)", Opt. Express 23, 21 (2015). CrossRef F. Hendricks, R. Patel, and V.V. Matylistsky, "Micromachining of bio-absorbable stents with ultra-short pulse lasers", Proc. SPIE 9355, 935502 (2015). CrossRef W.Y. Yeong et al. "Annealing of Biodegradable Polymer Induced by Femtosecond Laser Micromachining", Adv. Eng. Mater. 4, 12 (2010). CrossRef K. Stolberg et al. "IR and green femtosecond laser machining of heat sensitive materials for medical devices at micrometer scale", Proc. SPIE 8968, 89680E (2014). CrossRef F. Hendricks et al. "High aspect ratio microstructuring of transparent dielectrics using femtosecond laser pulses: method for optimization of the machining throughput", Appl. Phys. A 117, 1 (2014). CrossRef A. Antonczak et al. "Degradation of poly(l-lactide) under CO2 laser treatment above the ablation threshold", Polym. Deg. Stab. 109, 97-105 (2014) CrossRef B. Stepak et al. "The influence of ArF excimer laser micromachining on physicochemical properties of bioresorbable poly(L-lactide)", Proc SPIE 9736, 97361T (2016). CrossRef

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Abstract Abstract 4624 Mantle cell lymphoma (MCL) represents B-cell lymphoma derived from the mantle zone that surrounds normal germinal center follicles. Pathophysiology of this hardly curable disease involves t(11,14)(q13,q32) translocation which leads to upregulation of Cyclin D1(CCND1). Recently, microRNAs were demonstrated to significantly modify MCL pathogenesis (Jian-Jun Zhao et al. 2010) and therapy responsiveness (Jiang et al. 2010). In order to broaden our knowledge of regulatory pathways in MCL we searched for differentially expressed microRNAs and their differentially expressed mRNA targets between MCL samples and control samples. Samples consist of magnetically separated B cells derived from peripheral blood. We used 1) Microarray mRNA hybridization [Affymetrix Human Genome U133 Plus 2.0 Array, N(MCL)=5, N(control)=5] and 2) microRNA profiling [TaqMan® Array Human MicroRNA Card A v2.0 technology, N(MCL)=5, N(control)=5] followed by statistical analysis [limma (Smyth 2005)]. Differentially regulated targets of the deregulated microRNAs were selected from the databases involving both predicted (Betel et al. 2007) or confirmed (Hsu et al. 2010) target mRNAs. Among the most significant upregulated microRNAs (exceeding 10 fold) are miR-9, miR-124 and miR-183. We have found that upregulated miR-9 has confirmed downregulated target gene PRDM1 (PR domain zinc finger protein 1) which plays a role in B cell maturation (Turner et al. 1994) and may act as a tumor suppressor (Pasqualucci et al. 2006). Upregulation of miR-9 and downregulation of its targets was recently demonstrated in Burkitt lymphoma (Onnis A et al. 2010) and Hodgkin lymphoma (Nie K et al. 2008). Two additional upregulated microRNAs, miR-124 and miR-183, yet not associated with lymphomas, have downregulated target gene Integrin beta-1 (CD29) which regulates survival (f*ckumori et al. 2003). Among most significant downregulated microRNAs is miR-101 (FC=-7). Downregulated microRNA miR-101 has known oncogene N-Myc (MYCN) as upregulated confirmed target and DNA (cytosine-5)-methyltransferase 3A (DNMT3A) as upregulated target. Overexpression of a well known hematopoietic oncogene MYCN and epigenetic repressor DNMT3A may represent additional pathogenesis-related factors in MCL. Our data support importance of the candidate mechanisms involving microRNAs and their target programs in pathogenesis of MCL. They are currently extended on a larger patient cohort by analyzing expression and functional significance of the candidate microRNAs. (Grants: NT10310-3/2009, MPO FR-TI2/509, NPVII 2B06077, MSM 0021620806, LC 06044, SVV-2011-262507). Disclosures: No relevant conflicts of interest to declare.

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This study aim to attempt mapping out the Land Use or Land Cover (LULC) status of Regional Project Coordination Committee (RPCC) between 2009-2019 with a view of detecting the land consumption rate and the changes that has taken place using RS and GIS techniques; serving as a precursor to the further study on urban induced variations or change in weather pattern of the cityn Rangpur City Corporation(RCC) is the main administrative functional area for both of Rangpur City and Rangpur division and experiencing a rapid changes in the field of urban sprawl, cultural and physical landscape,city growth. These agents of Land use or Land cover (LULC) varieties are responsible for multi-dimensional problems such as traffic congestion, waterlogging, and solid waste disposal, loss of agricultural land. In this regard, this study fulfills LULC changes by using Geographical Information Systems (GIS) and Remote Sensing (RS) as well as field survey was conducted for the measurement of change detection. The sources of data were Landsat 7 ETM and landsat 8 OLI/TIRS of both C1 level 1. Then after correcting the data, geometrically and radiometrically change detection and combined classification (supervised & unsupervised) were used. The study finds LULC changes built-up area, water source, agricultural land, bare soil in a change of percentage is 17.23, 2.58, -9.94, -10.19 respectively between 2009 and 2019. Among these changes, bare soil is changed to a great extent, which indicates the expansion of urban areas is utilizing the land to a proper extent. Keywords: Urban expansion; land use; land cover; remote sensing; geographic information system (GIS); Rangpur City Corporation(RCC). References Al Rifat, S. A., & Liu, W. (2019). 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Abstract:

Biosurfactants are natural substances produced by several bacterial and fungal organisms that are amphiphilic and are extracellular (a part of the cell membrane). Biosurfactants can reduce the stress between solids and liquids on the surface and at the end. Biosurfactants have several properties, i.e. they are stable, less harmful, as well as readily degradable, and extremely eco-friendly. Biosurfactants also have a wide range of industrial uses because they are a versatile category of chemical substances. The principal justification for conducting such research was the isolation of possible biosurfactants containing bacteria. Sampling was performed for the isolation of bacteria producing biosurfactants from different oil-polluted sites That is to say, experiment for emulsification, test for oil spreading, test for drop collapse, and measure for hemolysis. The capability to produce biosurfactants was seen in 22 different isolates from polluted sites B1, B2, and B3. Through different biochemical tests and Gram staining, it was identified that isolated bacterial strains are Pseudomonas spp and that is Pseudomonas aeruginosa. The procedure used as characterizing biosurfactants was the TLC plate’s procedure, by using TLC plates process yellow dots emerged after spraying on silica gel plates with an throne and ninhydrin reagents. These yellow spots confirmed the presence and production of rhamnolipid in the biosurfactant. Hence, it was concluded that identified strains in the study can be helpful in the heavy metals, pesticides, and hydrocarbons bio-degradation and bioremediation. These may also be used as biological control agents to protect plants from various pathogens, resulting in improved crop yields. Introduction Biosurfactants are natural substances produced by several bacterial and fungal organisms that are amphiphilic and are extracellular (a part of the cell membrane) (Chen et al., 2007; Ghayyomiet al., 2012). Main purpose of the bio-surfactantsgeneration or production is a consequence of financial availability (Van Dyke et al., 1993 It is reported that almost 50 percent of the world's surfactants are used because of the need for cleaning agents as well as the rate of growth grows every day (Deleu and Paquot, 2004). Appropriate use of bio-surfactants will control environmental emissions what these are the most dangerous, constantly rising gradually and disrupting the routine maintenance of life every day. Awareness campaign initiatives have been introduced and also increase for environmental laws, various innovative approaches need to be implemented and even the issue of pollution focused entirely. Developing appropriate advanced technologies to help clear up chemicals and toxins from the ecosystem, like hydrocarbons (both inorganic and organic). Studies on biosurfactants are being launched by scholars and researchers with significant health issues like adverse environmental effects, air contamination, environmental change, and waste management (Makkar and Cameotra, 2002 Biosurfactants contribute to expanded demand for such microbial products as alternatives to chemical surfactants (Benatet al., 2000). Microbes seem to have the capability to degrade contaminants, but their biodegradation is limited leading to hydrophobicity, low solubility in water, and inadequate bioavailability, of such pollutants (Patil, et al., 2012). GhayyomiJazeh, Mishraet. al (2001) those bacteria that produce biosurfactants were isolated from the site of petroleum spills and afterward, 160 strains and as well as 59 strains were able to produce biosurfactants have shown better performance in a test for hemolysis of blood, and 45 strains with positive findings within oil spread experiment were applied in the laboratory to isolate and segregate the media cultured Banat process (Rahman et al., 2002) These were observed and researched that biosurfactants of Pseudomonas aeruginosa spp are most likely to disrupt the bonding of hydrocarbons like nonadecane, octa, Hexa, and hepta, in marine Water contaminated with oil spills up To approximately 47%, 53%, 73% and 60%(Abrar et al., 2020). Current study concluded that the isolated strain having the ability to degrade hydrocarbon as well as the ability to degrade the heavy metal. The strain also can protect the plant from various diseases. The present research found that the isolated strain is capable of degrading hydrocarbon while also being capable of degrading the heavy metal. As well as the strain does have the capability to defend plants from different diseases. Material And Methods Area of Study The investigation was conducted at HazaraUniversity(HU) Microbiology Laboratory, MansehraPakistan. Assemblage of Samples Thehomestay area of the city Mansehra Pakistan which is named as a township, where oil spills arose, oil spills soil samples were obtained as well as sampling from various Mansehra automobile workshops were also done. Sterilized bags of polythene were being used to collect samples of the soil, after thatthe sample was taken towards the Hazara University (HU) Mansehra Microbiology Laboratory to examine and extract bacterial strains that could develop biosurfactants. The soil temperature at the time of sample selection was around 30 ° C. The pH was also verified by Galvano science companies at the time of selection by pH meter, and the pH being reported was 7. Preparation of Media 15 x 100 mm Petri dishes were being used to prepare the media. Agar plates were thoroughly cleaned with water from the tap and then carefully covered in aluminum foil following cleaning then placed within autoclave at 121°C for about 15 min at 15 psi for sterilization. The nutrient agar which contains 0.5% NaCl, 0.3% beef extract, 0.5% peptone, and 1.5% agar, in 500 ml of distilled water, 14 g of the nutrient agar media (Merck) were dissolved. The nutrient level used mainly for the production of non-fastidious species. Nutrient agar is widely known as it's capable of growing a variety of bacteria types and provides nutrients required for the growth of bacteria. Upon sufficient dissolution of such nutrient agar in distilled water, these were then sterilized by autoclaving for 15 min at 15 psi in the autoclave and held at 121 °C Upon autoclaving, pouring of the media was done in laminar flow hood, and then packed and placed for yet more use in a fridge at 4°C. 2.4 Preparation of serial dilution The bacteria are isolated using the serial dilution process. During this process, 10 test tubes were taken and distilled water (9ml) was added in each tube. After that tubes were put for 15 minutes in the autoclave machine at 121°C. After that 1gm of a crude oil sample from the soil was added in a test tube containing distilled water. Further, 1 ml of the solution was taken from the first test tube and poured to the adjoining tubes for the preparation dilution as under . Afterward, 10μl of the solution was pipetted from both the dilution of and shifted for spread culture techniques, then incubated the plates at 37°C for 48hrs. Biosurfactants extraction Firstly, in nutrient broth solution theculture of bacteria was added and inoculated with oil, the bacterial colony was then incubated at the temperature of 25°C in a shaking incubator just for 7 days. Incubation after seven days of trembling. Thebacterial Crop was then taken and centrifuged at 5000rpm at temperature 4°C for 20minutes. Following centrifugation, the supernatant was collected and then mixed in the equivalent amount in Methanol: Chloroform. White sediment was then retained and collected for further use . Bacterial Colonies Isolation 1 g of the soil polluted with oil was diluted serially up to 106 dilutions.10 μl of 104 and 106 dilutions for spread culture were transferred to the MSM agar plates and nutrient agar. The plates were then incubated at 37°C for 48hrs. Twenty-two morphologically separate colonies were separated for further specific examination just after the incubation and processed by using the technique of streak plate. Screening of Isolates’ Biosurfactants Behavior To check the activity of biosurfactants produced by the bacterial species the following methods of screening were done. Hemolytic Activity of Biosurfactants for Erythrocytes Blood agar containing 5% of blood was prepared as after the fresh isolates were added and inoculated on blood agar plates, then the plates were taken and placed in the incubator at temperature 37°C for 48hrs (Rashediet al., 2005). Thereafter the observation of clear zone in the colonies indicated the existence of bacterial species that produce biosurfactants. This experiment was undertaken to control the ability of isolated bacteria to induce blood agar hemolysis. Three forms of hemolysis usually involve; alpha, beta, and hemolysis of the gamma. The agar underneath the species is dark greenish, then it is Alpha, the yellowish color produced in beta hemolysis and gamma hemolysis does not affect the bacterial sppwhichadded on the plates (Anandaraj and Thivakaran, 2010). Bio-surfactant identification with process of CTAB MSM (Mineral salt agar medium) with (2%) of glucose serving both as carbon source, (0.5 mg / ml) acetyl-tri-methyl-ammonium-bromide (CTAB), and methylene blue (MB: 0.2 mg/ml) are used to detect anionic bio-surfactants (Satpute et al., 2008). For this method, thirty microliters (30μl) of cell-free supernatant were added to each of the wells of the methylene blue agar plate that comprises of borer (4 mm in diameter). after that, the incubation of the plates was done for 48-72 hrs at 37°C. Just after incubation in each of the wells, a dark blue halo zone was being used to show the successful anionic bio-surfactant production. Table 1: Composition of MSM Media S. No Ingredients Amount (gm/L) I Potassium dihydrogen phosphate (KH2PO4) II Magnesium Sulfate (MgSO4) III Iron Sulfate (FeSO4) IV Sodium Nitrate (NaNO3) V Calcium Chloride (CaCl2) VI Ammonium Sulfate (NH4)2SO4 Technique for Spreading of Oil A sufficient number of isolated bacteria were inoculated into a solution of 100ml nutrient broth. Over 3 days, the culture was incubated at 37 ° C in a rotating shaker incubator (150 rpm). After that biosurfactants synthesis was checked in culture suspensions (Priya and Usharani, 2009; Anandaraj and Thivakaran, 2010). For this process, thirty milliliters (30ml) of distilled water was added in a Petri dish. In the middle of the distilled water, 1 milliliter (1ml) of diesel oil was added, and then a centrifuged twenty microliter (20μl) culture was introduced to the middle of a plate, which was isolated from oil spilled soil or local oily groundwater. The species producing the bio-surfactant displace the hydrocarbons and disperse it even in the water. Then it was calculated and analyzed within 1 mint (Ali et al., 2013). Technique for Drop collapse In this process, 96-wellsformed in each of the plates of nutrient agar. Afterwards, all the 96-wells of microliter plates was then filled withmineral oil of about 2ml. Then stabilized the plate at 37oC for 1 hour, after which the oil surface was filled with 5μl of supernatant culture. Therefore, the drop shape was taken to be observed on the oil surface after 1min. The drop which was collapsed, generated by the supernatant culture which is used to signify positive(+ive) outcome and the drops which stayed the same and displayed no changeindicates negative(-ive) outcome. And was taking distilled water as a control(Plaza et al., 2006). Emulsification index The emulsification index was calculated, as stated by the process followed by Cooper and Goldenberg (1981) In this process, 2 ml of kerosene oil was taken and inserted in each of the test tubes to the same amount of cell-free supernatant, and then hom*ogenized for 2 min in a vortex at high speed and allowed for 24 hours to stand. The emulsification steadiness was then determined after the 24 hours, and the emulsification value was estimated by measuring the emulsified layer height by the total liquid layer height, then multiplied by 100. Quantification for the Dry weight of Biosurfactants The bacterial colony was inserted and inoculated in the nutrient broth medium, followed by oil and centrifuged at 5000rpm and after that, the supernatant was clutched and treated with chloroform and methanol and mixed. The white colored deposits were taken and used for the furtherprocess of dry weight. Afterwards, took the clean Petri plate and determined the empty plate weight. Next, the sediment was poured onto Petri plates. Now, for the drying process the hot air oven was used and set the 100ºC of temperature for 30minutes and the plates were put in the oven. After the drying process, the plates were weighted again. The dry weight was calculated for the biosurfactants using the formula which described below: Selected strains Identification and their characterization Instead, various basic biochemical methods were used to identify the isolated bacterial strains. Various biochemical tests, such as Gram staining, Oxidase test, Urease test.Catalase test, Methyl red test, Motility test, Indole test, Starch hydrolysis, Citrate test, Spore staining, Gelatin hydrolysis. Then afterwards, for the preliminary characterization of the biosurfactant, the thin layer chromatography process was used. Physical characterization of the strains selected Gram staining First, on the slide, using the wire loop the bacterial pure culture was taken, and smear was prepared on the slide, and then a drop of purified water was applied. Then, the sterile loop or needle was correctly mixed the bacterial colony and purified water, then mixed up until it is somewhat turbid. Then, spirit lamp was used to fixed the bacterial smear on slide and cooled to room temperature. With this glass slide was loaded with solution of crystal violet and stood for 1minute anddistilled water was applied on slide. Meanwhile the slide was submerged for 1 minute with the iodine solution, and then flushed and rinsed with water. Therefore, decolorizer of about 1 to 2 drops(5 percent acetone and 95 percent alcohol) were added to the slide’s smear and stand for 30seconds, and then treated with water. After then slide was rinsed with safranin for 60seconds, and then treated with water anddry in air. Microscopic analysis was done with 100x objective lenses using emersion oil on smear. Cell morphology The isolates of the bacterial cell were gram stained on slides and then the slides were observed under the light microscope, showing the shape and color of the cells. Biochemical characterization of the selected strains Catalase test Aim of this study is to identify, evaluate and examine that, whether or not the microbes are capable of producing catalase enzymes, while catalase is a protective enzyme, i.e. catalase has the potential to protect against the lethal chemicals known as (H2O2). In this study a bacterial culture that was clarified overnight was used. This culture has been smeared on a glass slide, and 3 percent hydrogen peroxide (H2O2) has been applied and observed on smear. Effects have been observed for bubble formation. Citrate test This study was performed to check the amount or ingest the citrate as the carbon and energy supply for growth and metabolism. Medium containing bromothymol blue and sodium citrate as pH indicator, bacterial was introduced. Ammonium chloride is also present in this medium used as a nitrogen source. Results were noted with variations of color from green to blue. Urease test The capability of urease enzyme for degrading urea was calculated in this bacterial capacity test. Bacterial culture was taken and inoculated for 48 hours at 37 ° C in urease broth, and then color was observed. Methyl red test Through using the process known as mixed acid fermentation which is used to evaluate the bacteria's acid production. The bacterial culture was taken and introduced in the broth of MR-VP and then incubated for 3days at a temperature of 37°C. Two (2) to three (3) drops of Methyl red were added in the broth medium after the incubation period. The change in broth color was observed for final results after a few seconds. Indole test Through using the process to assess the bacteria 's capability to crash indole from tryptophane molecules. After the 24 hours of incubated, taken the fresh inoculum of bacteria and then inserted into the tryptone medium, 24 hours of incubation of about 30oC, 2ml of the tryptone broth medium was added into a sterile test tube. Kovac's reagent was taken to be added (few drops) in sterile test tube and stimulated for a few minutes, and variations of color were detected. Gelatin test It is the approach assess to figure out the use of enzymes known as gelatins from bacterial organisms that precipitate the gelatin. Fresh inoculum of bacteria was taken after 24 hours, and inserted into the media of gelatin agar. This was incubated for around 24 hours, so the temperature did not exceed 30 ° C. Media was observed after incubation time. Starch hydrolysis Several of the micro-organisms that use the starch as a carbon energysource. Therefore, this method has been used to assess whether or not bacteria may use starch as a source of carbon. The bacterial fresh inoculum was spread on the petri starch agar plates, and after that the plate was incubated for 24 hours andmaintained the temperature at 30 to 35 ° C, then gradually applying the supplements of iodine to the plates to flow the change, and then examining the plates. Preliminary characterization of the strains selected Experimental characterization of the bio-surfactant was performed by using the process of TLC (Anandaraj et al., 2010). On a silica gel plate, crude portion of the rudimentary bio-surfactant was separated using Methanol: Chloroform: water (CH3OH: CHCl3: H2O) in the ratio of as an eluent with a different color producing reagents. Ninhydrin reagent (0.5 g ninhydrin in 100ml anhydrous acetone) was used to find bio-surfactant lipopeptide as red spots and anthrone reagent (1 g anthrone in 5ml sulfuric acid combined with 95ml ethanol) as yellow spots to identify rhamnolipid bio-surfactant (Yin et al., 2008). Results and Discussion Isolation of bacteria At first, twenty-two (22) strains from a polluted soil sample were isolated from nutrient agar media.Mixed culture provided by these colonies, so they were taken and smeared on the plates of nutrient agar and then fresh inoculum was collected and stored at temperature of 4oC for the further analysis. Bio-surfactants (surface-active compounds)are formed by a variety of amphiphilic bacterial and fungal organisms that are extracellular (a part of the cellular membrane) (Chen et al., 2007). Screening of Isolated strains for biosurfactant producing colonies Different experiments were carried out to identify, isolate and screen bacteria that are capable of generating bio-surfactants and that is Oil spreading technique(OST), blood hemolysis test(BHT), CTAB test, Emulsification operation. There were twenty-two distinct isolates observed in the current research. And the B1, B2 and B3culture were taken and selected from the twenty-two (22) strains isolated from the polluted spot, which were found to produce biosurfactant. And the oil spreading technique showed promising results for these strains. And strain B2 showed a greater displacement of oil and this is 4 mm. Oil spreading method is quick and often easy to handle, and this technique requires no particular equipment, only a very small amount of sample is used. This approach can be applied when the production and quantity of biosurfactant is small (Plaza et al., 2006) and (Youssef et al., 2004) Only bacterial cultures have been allocated and screened for bacterial species that can generate or use biosurfactants. Just three (3) strainsamong them presented the best results.Those 3 strain,s (B1, B2 and B3) were selected as an additional analysis. Blood hemolysis test On the petri plates of blood agar, the . Isolated bacteriaof B1, B2 and B3 were taken andstreak at the temperature about 37°C for 48 hours. Strain B1 demonstrated β (Beta) hemolysis after the incubation cycle and B2 and B3strains demonstrated γ (Gamma) hemolysis. The B1 strain had an emulsification index of about 74 percent and that was very high as compared to 70 percent for B2 and about 53 percent for B3 respectively. Around the same time, B1 strain showed β (Beta) hemolysis and γ (Gamma) hemolysis was shown bystrains B2 and B3 on the platesof bloodagar. The β hemolysisshowed by the strain B1 in the blood agar test, and the strain B2 and B3 showed γ (Gamma) hemolysis. It is determined that 20 percent strains that are the bestproducer of rhamnolipid have not fully lysed the blood, because the ability of the producer strains capacity not be responsible for the hemolytic activity. According to many researchers, who have shown that this is not such an effective tool for biosurfactant detection due to many bioproducts that may also induce red blood cell lysis, that is not so sufficient to be the surface-active molecule (Youssef et al., 2004). (Rashedi and others, 2005). Table2 Blood Hemolysis Test CTAB agar plate test This test confirms the anionic biosurfactants development. After plate incubation at a temperature of 37 ° C for 72 hours, dark blue hollow zone was existedaround each of the B1 strains wells, which clearly indicated the positive (+ive) development of anionic Biofactant. In addition, the B1 and B2 strains showed positive (+ I ve) results and, in the CTAB analysis, the B3 strain was found to be negative (-ive). The growing microorganisms when secreted the anionic biosurfactants on the plates of CTAB (cetyl-tri-methyl-ammonium-bromide) and methylene blue, then as a result the dark blue-purple insoluble ion pairs formed on the plates. The halo zone around each of the colonies was developed that can recognize rhamnolipid production and that was dark blue in colour, and could correlate with production of rhamnolipid (Siegmund et al., 1991). As indicated in (Fig1) Fig1: B1 positive on CTAB agar plate Oil Spreading Technique The oil was displaced by B1, B2and B3 strains in this test strain and showed a zone that was so clear. The bacterial strains capable of developing biosurfactant were tested and separated from the sample of soil which was oil spilled and brought from the District of Mansehra, Pakistan and from automobile workshops of Mansehra. As shown in (Fig.2). Fig.2: Results of Oil Spreading by B1, B2 and B3Table 3;.Test for oil spreads Bacterial culture Formation of zone (mm) Readings B,1 B,2 B,3 Drop-collapse technique During this process the drop shape was observed at the oil surface. As seen in Fig 3, the collapsed drop was provided by the supernatant culture B1 , B2 and B3.. Emulsification index Emulsification stability was measured with the use of kerosene oilin this test, and then observed the results. Since this emulsification index was calculated by dividing the height of the emulsion layer by the total height of the liquid layer and then multiplying by 100, as shown in the formulation below. Emulsification index Emulsification stability was measured with the use of kerosene oilin this test, and then observed the results. Since this emulsification index was calculated by dividing the height of the emulsion layer by the total height of the liquid layer and then multiplying by 100, as shown in the formulation below. Fig 3: Result of Drop-collapse test Table 4: The activity of Biosurfactant emulsification Dry weight of bio-surfactants In this examination, white-colored sediment was collected. Then measured the weight of the sterile Petri plate which was empty in the first step. Then, the sediment was poured into plates. The plates were taken and weighted after 30 minutes of drying on a hot air oven, following the process of drying. The weight of biosurfactants (dry weight) was measured using the following formulations: Fig 4: Dry weight of biosurfactants Table: 5: Dry weight of the biosurfactants Bacterial Culture Weight of the plate (g) biosurfactant in The plate after drying (g) Dry weight of Biosurfactant (g) B,1 B,2 B,3 Identification of selected strains and their characterization Gram staining For structural applications, and stroke analysis gram staining method was used.(Fig.5) shows findings from the process of gram staining. Fig 5: Microscopic view of Gram staining Biochemical identification of bacterial strains and their characterization Specific biochemical studies were performed to identify the species for further recognition and characterization. The bio-surfactant producing microorganism was found to be Pseudomonas aeruginosa after conducting various characterizations and the biochemical tests(Eric Deziel et al., 1996), Which can be used to further analyze and study the industrial development of the biosurfactant. Rhamnolipid is also isolated and produced from the Pseudomonas aeruginosa species on the silica gel plate (Rashedi et al., 2005), a form of biosurfactants highly recommended for processes of bioremediation. All the findings collected from biochemical testing were labeled as Berge 's Manual and it revealed that the protected microorganism was (Pseudomonas aeruginosa). Results of biochemical test were tabulated in (Table.5) Table 6: Bacterial strain identification Tests B1 B2 B3 Gram staining Negative Negative Negative Oxidases Positive e Positive Positive Catalase Positive Positive Positive Indole Positive Negative Negative Citrate Positive Negative Negative Urease Negative Positive Negative Nitrate Positive Positive Positive Motility Positive Positive Positive Gelatin hydrolysis Positive Negative Negative Lactose Negative Positive Positive Methyl red Negative Positive Positive Voges Proskauer Negative Negative Negative Fig 6: Results of biochemical tests(A) Methyl red and Voges Proskauer tests (b) catalase tests (c) oxidase tests (d) indole tests (e) citrate tests (g) lactose tests (h) urease tests Preliminary bacterial strain’s characterization The plates showed yellow dots, when sprayed with anthrone reagent. It indicated the existence of biosurfactants of rhamnolipid in the organism on the plate of TLC as seen in theFig.7 Fig 7: Biosurfactant characterization by TLC Conclusion Biosurfactant development is exciting and perceptible across industries to clean up oil waste and pollutants, particularly in the ecosystem.Compared with chemical surfactants, the biosurfactants are less harmful. It plays an important role in defining the advantages and the importance of industrial applications. Therefore, it is not possible to disregard the growing role and importance of biosurfactants in environmental sustainability.Biosurfactant formulations which can be used for bacterial, fungal, and viral organisms as growth inhibitors. Such biosurfactant inhibition properties can make them components that are applicable to Numerous illnesses that are used as medicinal agents. Therefore it was decided that the described strain could be used as a potential source for heavy metal bioremediation pesticide and hydrocarbon polluted sites. And also used as shielding the plant from different pathogens, contributing to improved crop yields. There is no doubt that the biosurfactants are a multifunctional, advanced, versatile, long-lasting and updated type not only for the twenty-first century but beyond. Conflict of interest The authors declared that they have no conflict of interest and the paper presents their own work which does not been infringe any third-party rights, especially authorship of any part of the article is an original contribution, not published before and not being under consideration for publication elsewhere. References Ali, S.R.; Chowdhury, B.R.; Mondal, P. and Rajak, S. “Screening and characterization of biosurfactants producing microorganism from natural environment (Whey spilled soil)”. Nat. Sci. Res. 2013, 3(13), 34–64. 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Rahman.; S, McClean.; R, Marchant.; and I, M. Banat. “Rhamnolipid biosurfactants production by strains of pseudomonas aeruginosa using low-cost raw materials”. 2002, 18, 1277-1281. H.; Jamshidi, E.;Mazaheri, Assadi. M.; and Bonakdarpour, B. “Isolation and production of bio-surfactant from Pseudomonas aeruginosa isolated from Iranian southers wells oils”. Int. Environ. Sci. Tech. 2005, 2(2), 121–127 Satpute, S.K.; Bhawsar, B.D.; Dhakephalkar, P.K.; and Chopade, B.A. “Assessment of different screening methods for selecting bio-surfactant producing marine bacteria”. Indian J. Marine Sci. 2008, 37, 243–250. Shafeeq, M.; Kokub, D.; Khalid, Z. M.; Khan, A. M.; Malik, K. A. (1989). MIRCEN J. Appl. Microbiol. Biotech. 1989, 5, 505–510. Siegmund, I. and Wagner, F. “New method for detecting rhamnolipids excreted by Pseudomonas species during growth on mineral agar”. Tech. 1991, 5, 265–268. Van Dyke, M. I.; Couture, P.; Brauer, M.; Lee, H. and Trevors, J. T. "Pseudomonas aeruginosa UG2 rhamnolipid biosurfactants structural characterization and their use in removing hydrophobic compounds from soil". J. Microbiol. 1993, 39, 1071-1078. Yin, H.; J, Qiang.; Y, Jia.; J, Ye.; H,Peng.; H, Qin.; N, Zhang. B. “Characteristics of bio-surfactant produced by Pseudomonas aeruginosa S6 isolated from oil containing water”. Process Biochemistry. 2008, 44: 302–308. Youssef, H.; Duncan, El.; Nagle, P.; Savage, N.; Knapp, M.; McInerney, J. “Comparison of methods to detect biosurfactant production by diverse microorganisms”. Microbiol Methods. 2004, 56, 339-347.

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Objective: To detect the factors which affect competitiveness of the hydrocarbon sector from the study context. In order to generate a proposal for the improvement of that sector. Methodology. Through working with experts, it is possible to interrelate information that allows visualizing in a global way the study problem. This is achieved through the study of the context variables (six), with support from the structural analysis scheme that will generate the four potential areas where the conflict zone, power zone, zone of autonomous problems and exit zone are located, allowing the clear detection of the incident factors. Results. Given the previous scheme and with an in-depth analysis, we propose a model that generates the strengthening of the key factors (power zone), also we search for a way to stabilize the factors of the zone of conflict; all this will consolidate the work of the type of organizations studied, enabling them to be competitive in a globalized environment.

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We present new LA-ICP-MS detrital zircon U-Pb age and trace element data of the Late Mesozoic sedimentary sequences from the western Shandong and Tanlu fault zone, with the aim to constrain the depositional ages and sedimentary sources. The samples from the western Shandong have similar U-Pb age spectra, which can be divided into three major age groups, peaking age at circa 2,475–2,540 Ma, 1820–1870 Ma and 257–285 Ma, with minor Mesoproterozoic, Neoproterozoic and Paleozoic detrital zircon grains. The sample JN recovered from the Tanlu fault zone has an overwhelming majority of the Early Cretaceous detrital zircons with the age peak at circa 125 Ma, whereas the Archean and Paleoproterozoic detrital zircons are subordinate. The weighted average age of the youngest zircons show that the Santai Formation probably had begun deposition at circa 158 Ma and terminated deposition at circa 150 Ma, and the Tianjialou Formation of the Dasheng Group had begun deposition at circa 122 Ma. Our study indicates that the activity of the dinosaurs might occur during the Late Jurassic rather than the Cretaceous in the Shandong province. In addition, most detrital zircons of the studied samples are characterized by the high Th/U ratios and left-inclined REE patterns, revealing a magmatic origin. Morphologically, most detrital zircon grains characterized by angular to sub-rounded shapes indicate a middle-short distance transport from the source regions, whereas minor detrital zircon grains show rounded shapes, indicating a long-distance transport or multiphase recycling. According to detrital age populations in this study, combined with previously published data, we conclude that depositional provenances of the Santai Formation were mainly derived from the western Shandong and Jiao-Liao Belt, and minor detritus were derived from the northern part of the North China Craton and Xing-Meng orogenic belt. The sediments deposited in the Tanlu fault zone were mainly derived from the volcanic and subvolcanic rocks of the Qingshan period in the eastern Shandong, and subordinate depositional sources were from the Jiao-Liao and the basem*nt uplift of the western Shandong, with minor supplier being derived from the Yinshan-Yanshan orogenic belt. The detrital provenance of the Santai Formation indicates that extension of the eastern NCC occurred during the Late Jurassic. The Neoproterozoic detrital zircons play a minor role in the studied strata, indicating that the large sinistral movement of the Tanlu fault zone might have occurred at the Early-Middle Jurassic and formed a paleogeographic separation of the western Shandong and eastern Shandong (Sulu orogenic belt).

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The organizational culture of the zone, the micro and medium company does not have any kind of specialized technical advice, much less with the application of integral collaborative consulting The model is related in parallel with applied research, thus providing an alternative solution or guide for people who are going to be venturing into the development of Action- Research, whatever the field of application, and which is difficult to find the characteristics special, applied in the field of work, and above all, click on the results of that application. It can also be modified by its components, since it must have particularity that each company requires to operate or solve a problem at any level and what this entails, since it mainly focuses on Quality as a tool, not as an end; because the end of the first achievement of any organization will be just the certification to venture into new stages that the total quality demands.

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Modern scholarship on the medieval Sahara has focused on a handful of famous entrepôt sites that have their origins in the 8th century or later, and as a result we still understand very poorly the nature and extent of Saharan oasis settlement and agriculture in the golden age of Saharan trade. This article presents the first securely dated chronology for oasis development in the north-west Sahara based on three seasons of archaeological survey and a comprehensive radiocarbon dating programme in the Wadi Draa, Morocco. The Draa Valley contains some of the largest, most populous and most productive oases in the Sahara, as well as serving as an important travel corridor for trading caravans coming from West Africa to access the Atlas passes and reach Marrakech. Focusing on evidence from a large zone of abandoned oases on the Kasr Bounou Plain, this article demonstrates that while oasis agriculture and settlement was taking place between the 4th–8th centuries—well before the Muslim conquest of Morocco—there was a significant increase in settlement and agricultural exploitation from the 9th century. This phenomenon is marked by the appearance of substantial mudbrick settlements, along with irrigation and field systems, and is coterminous with the development of the medieval trading entrepôt of Sijilmasa. A settlement boom and significant investment in irrigated oasis agriculture occurred between the 11th and 13th centuries, contemporary with Almoravid and Almohad rule of the Draa, followed by a retraction and abandonment of much of the oasis by the 16th century. The new evidence from the Draa challenges the long-held belief that sedentarization and irrigated oasis agriculture were unique to the medieval period in the north-west Sahara. OPEN ACCESS CC BY-NC-ND

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Swordtip squid (Uroteuthis edulis) is a primary target species of the commercial fishery in the southern East China Sea (ECS), and they normally migrate to a quasi-permanent upwelling zone (called a cold dome) off northeastern Taiwan for spawning and growth during spring (March–May) and fall (October–December) in a year. We examined the connection of the variability in its standardized annual catch per unit effort (CPUE) during 2009–2017 in regard to the physical processes on the southern shelf of the ECS using temperature and wind observations from an isle north of Taiwan (Peng-jia-yu) as well as satellite sea surface temperature and absolute geostrophic velocity. The annual CPUE is positively correlated with the daily temperature anomaly at Peng-jia-yu in the cold dome in October of the previous year and April of the year. A warmer environment favors the recruitment and consequently the catch of the swordtip squid. During the spawning periods of the 9 years, the warm water carried by the Kuroshio frequently intruded atop the cold dome, which benefited the growth of the larvae and consequently helped maintain a certain value of the standardized annual CPUE. The anomalously low CPUE in 2012 and 2016 is attributed to the blocking of the Kuroshio intrusion due to cold and less salty China Coastal Water atop the cold dome in the spring spawning of 2012 and 2016. Based on the velocity strength in the cold dome and in a specified shelf region together with the daily temperature anomaly at Peng-jia-yu, an occupation intensity factor is used to evaluate the dominance of warm Kuroshio water and cold shelf water in the cold dome, which could help predict annual catches.

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AbstractThrough an analysis of oracle bone inscriptions relating to attacks on the northern and western borders of the Shang Kingdom by various ethnic groups living in the Northern Frontier Zone, this paper suggests that the members of northern chiefdoms such as the Qiong Fang, Tu Fang, or Fang Fang mainly lived in the mountainous areas of present-day western and northwestern Shanxi, northeastern Shaanxi, and northern Hebei Provinces. The paper analyzes the characteristics of northern frontier-style bronzes unearthed from this region and suggests to which cultures they may have belonged. Based on these suggestions and analyses, this paper discusses the northern-style bronzes unearthed from offering pits, sacrificial pits and tombs at the Yinxu Site and reveals that the northern-style bronzes frequently seen at Yinxu, especially the ones dating to the late stage of Phase I and Phase II of Yinxu Period (i.e., those dating to the reigns of Kings Wu Ding and Zu Jia), are a sign of frequent warfare between the Shang people and the ethnic groups inhabiting the Northern Frontier Zone, warfare having been the main form of the contact between them. Moreover, this paper discusses the significance of the communication of the Shang with these northern ethnic groups for establishing exchange between the Shang and the people in the Eurasian Steppe.

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Water temperatures of hot springs close to tectonic fault zones often show some variations before earthquakes, and analyses of earthquake precursors in hot springs have significant referential meaning for earthquake monitoring and forecasting. This study measured the concentration of major ions in water from the Hongshuilantang Hot Spring in 2017 and 2020. The ion composition was classified by hydrochemistry into the HCO3·SO4-Na chemical type. The composition of hydrogen and oxygen isotopes in the Hongshuilantang Hot Spring were located near the global meteoric water line (GMWL), indicating that the recharge source of the hot spring was meteoric water. The δD and δ18O values were not plotted on the Glogal Meteroric Water Line (GMWL), and there were some deviations, which suggested that hot spring water underwent water–rock interactions. Deep circulation water played an important role during the evolution process of thermal water. Water temperature showed a decreasing trend from October 2013 to June 2015 during the Rushan earthquake swarm in eastern China. Because of the occurrence of the earthquake swarm, we inferred that regional stress in this area began to be released, allowing continuous rebalancing. Free surface water appeared in some aquifers, and the seepage of low-temperature underground water into the upper aquifer led to a drop in water temperature in the hot spring. The Hongshuilantang Hot Spring and the epicenter of the Rushan earthquake swarm were located on the Muping–Jimo seismological fault zone, with the same seismotectonic system and some genesis relationships.

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H. Mutabilis (Cotton rose or confederate rose) is a deciduous shrub in the Malvaceae family, with ornamental, medicinal and edible values (Fan et al. 2015). In May to August 2020, 40.4% of potted plants of H. mutabilis were found to have root and stalk rot in Chengdu Botanical Garden (E104°7′28″, N30°45′57″). At first the leaves of affected H. mutabilis turned yellow and wilted, followed by the stem and root cortex became dark brown and rotten. Finally, the whole plant died within two months. Root and stem produced white mycelium when the humidity exceeded 90%. Samples taken from the lesions were surface disinfested for 3 min in 4% sodium hypochlorite, rinsed in sterile water and plated on potato dextrose agar (PDA), 35 single-spore cultures with similar morphology isolated from symptomatic tissues were obtained and subcultured. After seven days at 25°C in the dark, the mycelium of a representative culture MFR1 covered the entire plate surface (9 cm diameter). The aerial mycelium of cultures were white and fluffy at first and produced lavender pigment on the back of the cultures in the later stage. After seven days, the cultures produced abundant sickle-shaped macroconidia which have 3 to 5 septations and some oval or oblong microconidia which have 0 to 1 septation. Macroconidia 22.35~46.67 μm (mean 32.11 μm) in length and 4.32~7.72 μm (mean 5.21 μm) in width (n = 100). Microconidia 7.10~21.85 μm (mean 11.62 μm) in length and 2.76~6.84 μm (mean 4.20 μm) in width (n = 100). Based on these characteristics, isolates were tentatively identified as Fusarium sp. (Crous et al. 2021). Pathogenicity was tested on 1-year-old potted seedlings of H. mutabilis by root-zone irrigation inoculation in Sichuan Agricultural University (Jia et al.2019). Conidia suspension (1×107conidia/mL,collected from MFR1 )was poured into the soil along the plant roots. The same amount of distilled water was poured around the roots of the control plants. All inoculated and control plants were incubated in the greenhouse (about 25 ± 2°C). The experiment was performed three times. Within 25 days after inoculation, all plants inoculated with pathogens showed symptoms similar to those in the field, whereas the controls remained symptomless. The pathogen was reisolated from all inoculated plants, and the cultural and morphological characteristics were the same as those of the original isolate. After DNA extraction and PCR amplification, the translation elongation factor 1-alpha (TEF) and RNA polymerase II second largest subunit (RPB2) genes of a representative culture MFR1, were sequenced (O’Donnell et al. 2010) and deposited in GenBank (accession numbers OK334295 and ON316728, respectively). The TEF and RPB2 sequences were 99.7% and 99.39% identical to those of F. oxysporum (MN892354 and MZ198892). The result was confirmed by multilocus phylogenetic analysis. Through morphological identification and molecular analyses, the pathogen was identified as F. oxysporum. F. oxysporum is known to infect cotton (Dowd et al.2004), soybean (Ellis et al.2016) and banana (Fourie et al.2011) among other hosts, but it is the first report of F. oxysporum infecting H. mutabilis in China or worldwide. This disease seriously reduced the survival rate of H. mutabilis and may become an important reason to hinder the increase of H. mutabilis in potted seedlings stage. Moreover, the findings will provide theoretical basis to solve the bottleneck problem affecting the popularization and propagation of H. mutabilis.

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In this paper, we propose a new method for domain adaptation in Statistical Machine Translation for low-resource domains in English-Vietnamese language. Specifically, our method only uses monolingual data to adapt the translation phrase-table, our system brings improvements over the SMT baseline system. We propose two steps to improve the quality of SMT system: (i) classify phrases on the target side of the translation phrase-table use the probability classifier model, and (ii) adapt to the phrase-table translation by recomputing the direct translation probability of phrases. Our experiments are conducted with translation direction from English to Vietnamese on two very different domains that are legal domain (out-of-domain) and general domain (in-of-domain). The English-Vietnamese parallel corpus is provided by the IWSLT 2015 organizers and the experimental results showed that our method significantly outperformed the baseline system. Our system improved on the quality of machine translation in the legal domain up to 0.9 BLEU scores over the baseline system,… Keywords: Machine Translation, Statistical Machine Translation, Domain Adaptation References [1] Philipp Koehn, Franz Josef Och, Daniel Marcu, Statistical phrase-based translation, In Proceedings of HLT-NAACL, Edmonton, Canada, 2003, 127-133. [2] Yonghui Wu, Mike Schuster, Zhifeng Chen, Quoc V. Le, Mohammad Norouzi, Wolfgang Macherey, Maxim Krikun, Yuan Cao, Qin Gao, Klaus Macherey, Jeff Klingner, Apurva Shah, Melvin Johnson, Xiaobing Liu, Łukasz Kaiser, Stephan Gouws, Yoshikiyo Kato, Taku Kudo, Hideto Kazawa, Keith Stevens, George Kurian, Nishant Patil, Wei Wang, Cliff Young, Jason Smith, Jason Riesa, Alex Rudnick, Oriol Vinyals, Greg Corrado, Macduff Hughes and Jeffrey Dean, Google’s neural machine translation system: Bridging the gap between human and machine translation, CoRR, abs/1609.08144, 2016. [3] Luisa Bentivogli, Arianna Bisazza, Mauro Cettolo and Marcello Federico, Neural versus phrase-based machine translation quality: A case study, 2016. [4] Barry Haddow, Philipp Koehn, Analysing the effect of out-of-domain data on smt systems, In Proceedings of the Seventh Workshop on Statistical Machine Translation, 2012, 422-432. [5] Boxing Chen, Roland Kuhn and George Foster, Vector space model for adaptation in statistical machine translation, Proceedings of the 51st Annual Meeting of the Association for Computational Linguistics, 2013, pp. 1285-1293. [6] Daniel Dahlmeier, Hwee Tou Ng, Siew Mei Wu4, Building a large annotated corpus of learner english: The nus corpus of learner english, In Proceedings of the NAACL Workshop on Innovative Use of NLP for Building Educational Appli-cations, 2013. [7] Eva Hasler, Phil Blunsom, Philipp Koehn and Barry Haddow, Dynamic topic adaptation for phrase-based mt, In Proceedings of the 14th Conference of the European Chapter of The Association for Computational Linguistics, 2014, pp. 328-337. [8] George Foster, Roland Kuhn, Mixture-model adaptation for smt, Proceedings of the Second Workshop on Statistical Machine Translation, Prague, Association for Computational Linguistics, 2007, pp. 128-135. [9] George Foster, Boxing Chen, Roland Kuhn, Simulating discriminative training for linear mixture adaptation in statistical machine translation, Proceedings of the MT Summit, 2013. [10] Hoang Cuong, Khalil Sima’an, and Ivan Titov, Adapting to all domains at once: Rewarding domain invariance in smt, Proceedings of the Transactions of the Association for Computational Linguistics (TACL), 2016. 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[16] Adam Berger, Stephen Della Pietra, and Vincent Della Pietra, A maximum entropy approach to natural language processing, Computational Linguistics, 22, 1996. [17] 18Santanu Pal, Sudip Naskar, Josef Van Genabith, Uds-sant, English-German hybrid machine translation system, In Proceedings of the Tenth Workshop on Statistical Machine Translation, Lisbon, Portugal, September, Association for Computational Linguistics, 2015, pp. 152-157. [18] Louis Onrust, Antal van den Bosch, Hugo Van hamme, Improving cross-domain n-gram language modelling with skipgrams, Proceedings of the 54th Annual Meeting of the Association for Computational Linguistics, Berlin, Germany, 2016, pp. 137-142. [19] Mark Aronoff, Kirsten Fudeman, What is morphology, V 8. john wiley and sons, 2011. [20] Laurence C. Thompson, The problem of the word in vietnamese, In journal of the International Linguistic Association 19(1) (1963) 39-52. https:// doi.org/1080/00437956.1963.11659787. [21] Binh N. Ngo, The Vietnamese language learning framework, Journal of Southeast Asian Language Teaching 10 (2001) 1-24. [22] Le Hong Phuong, Nguyen Thi Minh Huyen, Azim Roussanaly, Ho Tuong Vinh, A hybrid approach to word segmentation of vietnamese texts, 2008. [23] Philipp Koehn, Hieu Hoang, Alexandra Birch, Chris Callison-Burch, Marcello Federico, Nicola Bertoldi, Brooke Cowan, Wade Shen, Christine Moran, Richard Zens, Chris Dyer, Ondřej Bojar, Alexandra Constantin, Evan Herbst, Moses: Open source toolkit for statistical machine translation, In ACL-2007: Proceedings of demo and poster sessions, Prague, Czech Republic, 2007, pp.177-180. [24] Franz Josef Och, Minimum error rate training in statistical machine translation, In Proceedings of ACL, 2003, pp.160-167. [25] Andreas Stolcke, Srilm - an extensible language modeling toolkit, in proceedings of international conference on spoken language processing, 2002. [26] Papineni, Kishore, Salim Roukos, Todd Ward, WeiJing Zhu, Bleu: A method for automatic evaluation of machine translation, ACL, 2002. [27] G. Klein, Y. Kim, Y. Deng, J. Senellart, A.M. Rush, OpenNMT: Open-Source Toolkit for Neural Machine Translation. ArXiv e-prints. [28] Pratyush Banerjee, Jinhua Du, Baoli Li, Sudip Kr. Naskar, Andy Way and Josef van Genabith, Combining multi-domain statistical machine translation models using automatic classifiers, In Proceedings of AMTA 2010., 2010.

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